Wnt inhibition decreases olig2+ cell vessel migration and brain endothelial cell signaling. (A)Tg(olig2:dsRed;fli1a:eGFP) animals were treated for 72 h from 3 to 6 dpf with DMSO vehicle, the Wnt inhibitor, XAV939, or the Vegf inhibitor, AV951. olig2+ cell-vessel interactions were quantified after 72 h of each treatment. A one-way ANOVA with Tukey’s Multiple comparisons test was performed (****p < 0.0001). Error bars represent standard error of the mean. n = 7–8 animals per group. (B) Quantification of individual cells from animals treated in panel (A). A one-way ANOVA with Tukey’s Multiple comparisons test was performed (**p < 0.005). Error bars represent standard error of the mean. n = 6–7 animals per group. (C) Quantification of the total number of olig2+ cell-vessel interactions divided by total number of olig2+ cells per condition. A one-way ANOVA with Tukey’s Multiple comparisons test was performed (*p < 0.005). Error bars represent standard error of the mean. n = 10 animals per group. (D) Representative live confocal images of Tg(glut1b:mCherry) animals treated with either DMSO vehicle control or XAV939 from 3 to 6 dpf. The right panels are the zoomed in areas from the corresponding white boxes in the left panels. Scale bar = 20 μm. (E) Quantification of glut1b:mCherry fluorescence after 72 h of XAV939 treatment. A two-tailed, unpaired t-test was performed (***p < 0.001). Error bars represent standard error of the mean. n = 9–11 animals per group.
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