FIGURE

FIGURE 2

ID

ZDB-FIG-210718-9

Publication

Umans et al., 2021 - Using Zebrafish to Elucidate Glial-Vascular Interactions During CNS Development

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FIGURE 2

Zebrafish migratory olig2+ glia expand during CNS angiogenesis and co-opt the developing vascular network. (A–F) Daily live confocal images of the Tg(olig2:dsRed) line (red) crossed to the Tg(fli1a:eGFP)^y1 line (green) from 1 to 6 dpf. Red olig2+ glia move out from the ventral brain and migrate along green blood vessels during brain development. Scale bar = 50 μm. (G) A representative maximum intensity projection of a single olig2+ cell (red) sitting on a vessel (green) in the developing zebrafish forebrain (left panel). The orthogonal horizontal (XZ) and vertical (YZ) views (middle and right panel) from the crosshairs where the olig2+ cell sits on a vessel. The orthogonal views are displayed above their corresponding fluorescence line profiles whereby an arrow denotes an olig2+ -vessel interaction and an asterisk shows signal from a nearby vessel without an olig2+ cell. Scale bar = 10 μm. (H)olig2+-vessel interactions quantified from 3 to 6 dpf. A one-way ANOVA with Tukey’s Multiple comparisons test was performed. *p ≤ 0.05, **p ≤ 0.005, ****p ≤ 0.0001. Error bars represent standard error of the mean. n = 7–11 per group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol