ZFIN ID: ZDB-FIG-210708-25
Mikdache et al., 2021 - Rgs4 is a regulator of mTOR activity required for motoneuron axon outgrowth and neuronal development in zebrafish. Scientific Reports   11:13338 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Figure 3

Rgs4 is required for neuronal development in the PLLg and spinal cord. (AD), single planes of confocal microscopy images representing the PLLg following HuC labelling at 48 and 72 hpf in WT (A,C) and MZrgs4 (B,D). Scale bars = 5 μm. (E) Graph showing the number of neurons within the PLLg in WT (average of 56.70 ± 1.43, n = 10 at 48 hpf; average of 71.17 ± 1.25, n = 12 at 72 hpf) and MZrgs4 embryos (average of 29.14 ± 1.71, n = 14 at 48 hpf; average of 52.14 ± 1.33, n = 21 at 72 hpf) at 48 and 72 hpf. (F) Quantification of the number of neurons in the PLLg following rgs4 mRNA injection in MZrgs4 mutants at 48 hpf (average of 54.89 ± 1.01 neurons in WT, n = 9; average of 28.65 ± 1.43 neurons in MZrgs4, n = 17; average of 53.15 ± 1.13 neurons in MZrgs4 + rgs4mRNA, n = 13). (G,H) single planes of confocal microscopy images of Tg(HuC:gfp) (G) and Tg(HuC:gfp)/MZrgs4 (H) at 48 hpf. Scale bar = 20 μm. (I) Quantification of the number of HuC:gfp + cells within the same area of the spinal cord in WT (average of 129 ± 2.70 gfp + cells, n = 12) and MZrgs4 (average of 110.7 ± 2.07 gfp + cells, n = 12) at 48 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg standard conditions Long-pec spinal cord neuronal cell body IFL
rgs4kbc2/kbc2; knu3Tg standard conditions Long-pec spinal cord neuronal cell body IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT standard conditions Long-pec posterior lateral line ganglion neuronal cell body
IHC Protruding-mouth posterior lateral line ganglion neuronal cell body
IHC rgs4kbc2/kbc2 standard conditions Long-pec posterior lateral line ganglion neuronal cell body
IHC Protruding-mouth posterior lateral line ganglion neuronal cell body
Phenotype Details
Fish Conditions Stage Phenotype
rgs4kbc2/kbc2 standard conditions Long-pec posterior lateral line ganglion has fewer parts of type posterior lateral line ganglion neuron, abnormal
Protruding-mouth posterior lateral line ganglion has fewer parts of type posterior lateral line ganglion neuron, abnormal
rgs4kbc2/kbc2; knu3Tg standard conditions Long-pec spinal cord has fewer parts of type spinal cord neuron, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.