FIGURE

Figure 1

ID

ZDB-FIG-210708-23

Publication

Mikdache et al., 2021 - Rgs4 is a regulator of mTOR activity required for motoneuron axon outgrowth and neuronal development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Characterization of rgs4 mutant. (A) Schematic representation of the rgs4 genomic locus. The extended region on the exon 2 represents the sequence targeted by the CRISPR/Cas9 system. Red: sgRNA binding site. Blue: PAM sequence. Rgs4⁺ corresponds to the wild-type allele; rgs4⁻ is the loss-of-function allele used in this study. Dashes represent the 2 base pairs deletion. (B) Schematic of the wild type Rgs4 protein (Rgs4⁺) and the mutated Rgs4 protein (Rgs4⁻). The 215 amino acid (aa) long Rgs4⁺ protein contains the RGS domain from amino acid 71 to 183. In rgs4^−/− mutant fish, the 2 base pairs deletion results in a frame shift from the amino acid 28 generating a premature STOP codon at the level of the amino acid 64. (C) Lateral views of WT and MZrgs4 embryos at 48 hpf; black arrow designates a slight heart oedema observed in MZrgs4 embryos. Scale bar = 200 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rgs4^kbc2/kbc2
Observed In:	heart
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.