FIGURE

Figure 7

ID

ZDB-FIG-210708-30

Publication

Mikdache et al., 2021 - Rgs4 is a regulator of mTOR activity required for motoneuron axon outgrowth and neuronal development in zebrafish

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Figure 7

Loss of rgs4 function alters mTOR signaling in neural cells (A) Immunoblotting of lysates from zebrafish embryos at 20 hpf. S6 and p-S6 amounts were normalized to α-tubulin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. No significant difference was observed between the two groups. Immunoblotting of lysates from zebrafish embryos at 48 hpf. S6 and p-S6 amounts were normalized to β-actin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. MZrgs4 embryos show a significant decrease in the amount of p-S6/S6 in comparison to WT. (B) Immunoblotting of neural lysates from zebrafish embryos at 48 hpf. S6 and p-S6 amounts were normalized to β-actin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. MZrgs4 embryos showed a significant decrease in the amount of p-S6/S6 in comparison to WT.

Expression Data

Antibodies:	Ab1-rps6 Ab3-rps6
Fish:	WT rgs4^kbc2/kbc2
Anatomical Terms:	nervous system whole organism
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rgs4^kbc2/kbc2
Observed In:	nervous system nervous system TOR signaling whole organism whole organism TOR signaling
Stage Range:	20-25 somites to Long-pec

Phenotype Detail

Acknowledgments

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