Changes in the expression of lysosomal genes in Neu1-KO zebrafish. mRNA levels of ctsa (a), glb1 (b), galns (c), tfeb (d), lamp1a (e), and lamp1b (f) in WT and Neu1-KO zebrafish. Each level of gene expression in Neu1-KO zebrafish is relative to the value in WT fish. n = 7 for each group. (g) Lysates of zebrafish brains were applied for western blotting using anti-lamp1 and anti-β actin antibodies. Quantitative analyses of the intensities of Lamp1 and β-actin bands were carried out and results are presented as Lamp1/β-actin. (h–j) WT zebrafish were exposed to alarm substances and then the neu1 (h) and npy mRNA levels (i) were estimated by real-time PCR. Lysates of zebrafish brains were applied for western blotting using anti-lamp1 and anti-β actin antibodies (j). Quantitative analyses of the intensities of Lamp1 and β-actin bands were carried out and results are presented as Lamp1/β-actin. n = 6 for each group. Results are shown as means ± standard deviation. All values were compared using Mann–Whitney U test. n.s., not significant.
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