FIGURE

Figure 3

ID

ZDB-FIG-210707-20

Publication

Shan et al., 2021 - Identification and functional characterization of a fish-specific tlr19 in common carp (Cyprinus carpio L.) that recruits TRIF as an adaptor and induces ifn expression during the immune response

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Figure 3

Localization of CcTlr19.A For CcTlr19 colocalization with the endoplasmic reticulum, EPC cells were transiently transfected with Tlr19-GFP. After 24 h, the cells were fixed with 4% paraformaldehyde, stained with ER-Tracker, and then treated with Cy3-conjugated (red) goat anti-rabbit IgG secondary antibody (Ab). The red signal represents the ER, the green signal indicates CcTlr19, and blue staining indicates the nucleus. For CcTlr19 colocalization with endosomes, EPC cells transiently co-transfected with Tlr19-FUGW, Rab5-GFP (early endosome marker) B or Rab7-GFP (late endosome marker) C, and the cells were stained with mouse anti-FLAG antibody (Ab). The cells were treated with Cy3-conjugated secondary Ab and DAPI and then measured by immunofluorescence confocal microscopy. Red signals represent overexpressed CcTlr19, green signals display overexpressed endosomes, and blue staining indicates the nucleus.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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