Fig 3

The potassium channel subunit Kv1.1 regulates the magnitude of the startle response.

(A) Gene structure for kcna1a with the missense mutation from the screen noted (p181). The CRISPR 7 bp insertion allele is also noted (p410). (B) Protein structure for Kv1.1, which is encoded by kcna1a, on the plasma membrane, with the predicted amino acid change from the screen allele (p181) indicated. The insertion in the CRISPR allele (p410) causes a frame shift in the sequence that results in a premature stop at amino acid 210. In contrast to siblings (C-G), kcna1a mutants (H-L) display an exaggerated startle response, with an increased bend and counter bend angle (I,J), resulting in fish swimming in a “figure eight” (K). Blue bar = latency in G,L. (M-O) Kinematic parameters of the acoustic startle response in kcna1a sibling and mutants (homozygotes of the screen mutation, p181/p181, transheterozygotes of the screen mutation and CRISPR, p181/p410, and homozygotes of the CRISPR mutation, p410/p410). Each point represents average of ten trials for an individual fish. n≥17 larvae, **p<0.001 (one-way ANOVA with Tukey correction for multiple comparisons).