FIGURE

Fig 7

ID

ZDB-FIG-210623-20

Publication

Meserve et al., 2021 - A forward genetic screen identifies Dolk as a regulator of startle magnitude through the potassium channel subunit Kv1.1

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Fig 7

Kv1.1 is expressed in motor neurons and V2a interneurons.

(A) Kv1.1 (magenta:A; grey:A’) localizes to motor axons expressing hb9:GFP (green:A; grey:A”). (B-G) Kinematic parameters of the acoustic startle response in (B-D) sibling, ryr1b single mutants, dolk single mutants, and ryr1b^-/-;dolk^-/- double mutants, and (E-G) sibling, kcna1a single mutants, prdm12b single mutants, and kcna1a^-/-;prdm12b^-/- double mutants. Each point represents average of ten trials for an individual fish. n≥6 larvae, *p<0.04 **p<0.0001 (one-way ANOVA with Tukey correction for multiple comparisons). (H-I) Kv1.1 (magenta:H,I; grey:H’,I’) localizes to (H) V2a interneuron axons expressing chx10::GFP (green:H,I; grey:H”,I”) in the ventral spinal cord but not (I) V2a interneuron soma. (J) In dolk mutants, Kv1.1 (magenta:J; grey:J’) localizes to V2a interneuron soma expressing chx10::GFP (green:J; grey:J”). Scale bars = 50 μM.

Expression Data

Genes:	GFP mKate
Antibody:	Ab3-kcna1
Fish:	p418Tg nns18Tg; nns19Tg dolk^p420/p420; nns18Tg; nns19Tg
Anatomical Terms:	CiD axon CiD cell body motor neuron axon
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dolk^p420/p420 kcna1a^p181/p181 prdm12b^p419/p419 ryr1b^p414/p414 dolk^p420/p420; nns18Tg; nns19Tg dolk^p420/p420; ryr1b^p414/p414 kcna1a^p181/p181; prdm12b^p419/p419
Observed In:	CiD cell body startle response
Stage:	Day 5

Phenotype Detail

Acknowledgments

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