Systematic identification of mRNA localization patterns.a Heatmap representation of z-score normalized expression per section in a zebrafish one-cell stage embryo. Genes on the y-axis as sorted into profiles 1–50 by SOM (self-organizing map), spatial position in the embryo on the x-axis. b Vegetally localized genes per sample (profiles 48–50). c Expression correlation of vegetally localized genes between two replicates. Genes on the axes are only detected in one sample. d Comparison of tomo-seq and whole-mount in situ hybridization for selected newly described vegetally localized genes, as well as the animally localized gene exd2. Scale bars are 200 µm.
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