Tracking the fate of maternal transcripts by scSLAM-seq.a Fold change enrichment of maternal vegetally localized genes in the different cell types versus all other cells (color code as in Fig. 3d). Genes with an average expression <0.1 transcripts/cell were excluded from this analysis. Red bars represent mean values. b Deconvolution of the bimodal distribution of vegetally localized genes in PGCs (black dashed line) into two normal distributions (light and dark blue). The mean value of the dark blue distribution is significantly higher than that of a randomly sampled distribution (mgray = 0.4, mdark blue = 1.52, p value = 1.7 × 10−4, Welch’s t test, one-sided). c Average expression of most highly enriched genes in PGCs in different cell types (color code as in Fig. 3d). d Unlabeled RNA expression of established germ cell markers on a UMAP representation.
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