FIGURE

Figure 5.

ID

ZDB-FIG-210512-32

Publication

Zhang et al., 2021 - Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency

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Figure 5.

Western blotting analysis of mitochondrial proteins. (A, C) Twenty micrograms of total proteins from mutant and WT zebrafish were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with antibodies for 5 subunits of OXPHOS (mitochondrion-encoding Nd1, Co2 and Atp8 and nucleus-encoding Sdha and Atp5a), Tufm, Tfam and Tom20 as a loading control. Quantification of levels of OXPHOS subunits (B) and other mitochondrial proteins (D). Average contents of Nd1, Co2, Atp8, Atp5a, Sdha, Tufm and Tfam were normalized to the average content of Tom20 in mutant and WT zebrafish. The values for the mutant zebrafish are expressed as percentages of the average values for the WT zebrafish. The calculations were based on three independent determinations. Graph details and symbols are explained in the legend to Figure 2.

Expression Data

Genes:	mt-co2 mt-nd1 sdha tfam tufm
Fish:	AB mto1^{zf3385/zf3385} (AB) mto1^zf3385/+ (AB)
Anatomical Term:	whole organism
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mto1^{zf3385/zf3385} (AB) mto1^zf3385/+ (AB)
Observed In:	whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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