FIGURE

Fig. 3

ID

ZDB-FIG-210427-13

Publication

Lu et al., 2021 - Calycosin attenuates doxorubicin-induced cardiotoxicity via autophagy regulation in zebrafish models

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Fig. 3

Effect of CA on autophagy in adult zebrafish DIC model. (A) Representative western blot showing changes in LC3II protein levels in the heart after DOX and CA treatment. Baflomycin A1 (30 nM) was administered 4 h before sacrifice the fish to assess autophagic flux changes. (B, C) Quantification of relative amounts of LC3II. n = 3 hearts per group on each time point. Bars represent mean ± SD. (D, E) Representative images and quantification of sectioned ventricles of Tg(GFP-Lc3) fish at 56 dpi with or without BafA1 treatment. Arrows: Lc3 aggregating dots formed during autophagy. (F) Relative RNA expression degree of atg7 in indicated group. *P < 0.05, **P < 0.01.

Expression Data

Gene:	atg7
Antibody:	Ab11-map1lc3
Fish:	WT
Conditions:	chemical treatment by environment: bafilomycin A1 chemical treatment by injection: doxorubicin chemical treatment by environment: bafilomycin A1, chemical treatment by injection: doxorubicin chemical treatment by environment: calycosin, chemical treatment by injection: doxorubicin chemical treatment by environment: bafilomycin A1, chemical treatment by injection: doxorubicin
Anatomical Terms:	cardiac ventricle heart
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment by environment: bafilomycin A1 chemical treatment by injection: doxorubicin chemical treatment by environment: bafilomycin A1, chemical treatment by injection: doxorubicin chemical treatment by environment: calycosin, chemical treatment by injection: doxorubicin chemical treatment by environment: bafilomycin A1, chemical treatment by injection: doxorubicin
Observed In:	cardiac ventricle heart
Stage:	Adult

Phenotype Detail

Acknowledgments

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