Fig. 4
- ID
- ZDB-FIG-210427-14
- Publication
- Lu et al., 2021 - Calycosin attenuates doxorubicin-induced cardiotoxicity via autophagy regulation in zebrafish models
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Effect of CA on cell autophagy. (A) Effects of DOX (1 and 10??M) and CA (5, 50, 100, and 300??M) alone on viability of H9C2 cells at 24?h by MTT assay (n?=?3). (B) Atg7 was confirmed knockdown via RT-PCR in HeLa cells. (C) Quantitative analysis of yellow puncta (autophagosomes) and red puncta (autolysosomes). Data are expressed as mean?±?SD. *P?0.05 (D) Wild-type (Control) and atg7-knockout (SiRNA-atg7) HeLa cells were transiently transfected with pBABE-puro mCherry-EGFP-LC3B plasmid and then untreated or treated with CA (50??M) for 24?h. Representative images showing EGFP-LC3 and mCherry-LC3 puncta. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) |