|
Fig. 3 Effect of CA on autophagy in adult zebrafish DIC model. (A) Representative western blot showing changes in LC3II protein levels in the heart after DOX and CA treatment. Baflomycin A1 (30 nM) was administered 4 h before sacrifice the fish to assess autophagic flux changes. (B, C) Quantification of relative amounts of LC3II. n = 3 hearts per group on each time point. Bars represent mean ± SD. (D, E) Representative images and quantification of sectioned ventricles of Tg(GFP-Lc3) fish at 56 dpi with or without BafA1 treatment. Arrows: Lc3 aggregating dots formed during autophagy. (F) Relative RNA expression degree of atg7 in indicated group. *P < 0.05, **P < 0.01.