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Figure 6

ID: ZDB-IMAGE-210307-96
Publication: Li et al., 2021 - Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing
All Figures: Figures for Li et al., 2021

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Figure Caption

Figure 6

The defective retinal development in prpf31 mutants can be rescued by injection of wild-type but not RP mutant PRPF31 mRNA. (A,B) The retina phenotype of the siblings and mutants injected with human PRPF31 wild-type mRNA or RP mutant mRNA. BF, bright field; Islet1, neurons marker; pH3, M-phase marker; TUNEL, apoptosis marker; phalloidin, labeled the lamination of the retina. (C−F) The statistical data show the numbers of specific positive cells in (A), (B). Note that wild type mRNA can significantly rescued the deficient phenotypes in mutant retina, while p.Lys257fs*277 mutant mRNA has almost no effect, the p.Arg372Glnfs*99 mutation seems to have a negative effect on siblings and mutants at 60 hpf. The number of embryos used in each assay was 6. Scale bar, 100μm.

Acknowledgments

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