FIGURE

Figure 5

ID

ZDB-FIG-210303-5

Publication

Hong et al., 2021 - Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

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Figure 5

Effect of UA extract on the regulation of the nuclear factor- (NF-) κB pathway. Cells were treated with the indicated concentrations of UA extract for 1 h, followed by induction with LPS (0.5 μg/mL) for 30 min. Equal amounts of protein were analyzed using specific antibodies against p-IκB and p65. Levels of p-IκB and p65 protein in (a) cytosolic and (b) nuclear fractions measured by western blotting. Three independent experiments were performed, and the data are presented as the mean ± SEM. *P<0.05 and **P<0.01 compared to the cells treated with LPS alone. Expressions of GAPDH and PCNA were used as internal controls for western blot analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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