FIGURE

Figure 2

ID

ZDB-FIG-210303-2

Publication

Hong et al., 2021 - Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

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Figure 2

Inhibitory effects of UA extract on nitric oxide (NO) production and inducible-nitric oxide synthase (iNOS) expression in LPS-stimulated RAW 264.7 cells. (a) Cells were pretreated with the indicated concentrations of UA extract for 1?h prior to incubation with LPS (0.5??g/mL) for 24?h. The nitrite levels in the culture medium were measured by the Griess reaction. Three independent experiments were performed, and the data are presented as the mean ± SEM. *P<0.05 and **P<0.01 compared to the cells treated with LPS alone. (b) Cells were pretreated with UA extract for 1?h and then stimulated with LPS for 6?h. ?-Actin expression was used as an internal control for quantitative RT-PCR. (c) Cells were pretreated with UA extract for 1?h and then induced with LPS for 24?h. Ten cell lysates were prepared, and the iNOS protein levels were analyzed on western blots. Expression of ?-actin was used as an internal control for western blot analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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