PUBLICATION
Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model
- Authors
- Hong, J.M., Kim, J.E., Min, S.K., Kim, K.H., Han, S.J., Yim, J.H., Park, H., Kim, J.H., Kim, I.C.
- ID
- ZDB-PUB-210302-10
- Date
- 2021
- Source
- BioMed Research International 2021: 8812090 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/chemistry
- Anti-Inflammatory Agents/pharmacology*
- Ascomycota/chemistry*
- Complex Mixtures/chemistry
- Complex Mixtures/pharmacology*
- Disease Models, Animal
- Inflammation/chemically induced
- Inflammation/drug therapy
- Inflammation/metabolism
- Inflammation/pathology
- Lichens/chemistry*
- Lipopolysaccharides/toxicity*
- Methanol/chemistry
- Mice
- RAW 264.7 Cells
- Zebrafish
- PubMed
- 33644231 Full text @ Biomed Res. Int.
Citation
Hong, J.M., Kim, J.E., Min, S.K., Kim, K.H., Han, S.J., Yim, J.H., Park, H., Kim, J.H., Kim, I.C. (2021) Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model. BioMed Research International. 2021:8812090.
Abstract
Umbilicaria antarctica (UA) is a member of the family Umbilicariaceae. To the best of our knowledge, no studies on its anti-inflammatory effects have been reported yet. In the present study, we examined its ability to suppress inflammatory responses and the molecular mechanisms underlying these abilities using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells and a zebrafish model of inflammation. We investigated the effects of UA on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW 264.7 cells. To explore the anti-inflammatory mechanisms of UA, we measured the mRNA and protein expression of proinflammatory mediators in LPS-stimulated RAW 264.7 cells using quantitative RT-PCR and western blot analyses, respectively. UA significantly inhibited the production of NO, PGE2, interleukin- (IL-) 6, and tumor necrosis factor- (TNF-) α in the LPS-stimulated RAW 264.7 cells. It also suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor- (NF-) κB activation in LPS-stimulated RAW 264.7 cells and tail pin-cutting-induced zebrafish model. Collectively, these findings indicate that UA significantly inhibits LPS-stimulated inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. Overall, our results demonstrate that UA extract exerts strong anti-inflammatory activities in in vitro and in vivo models and suggest that UA may be an effective novel therapeutic agent for the treatment of inflammatory diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping