|
Figure 5 Effect of UA extract on the regulation of the nuclear factor- (NF-) κB pathway. Cells were treated with the indicated concentrations of UA extract for 1 h, followed by induction with LPS (0.5 μg/mL) for 30 min. Equal amounts of protein were analyzed using specific antibodies against p-IκB and p65. Levels of p-IκB and p65 protein in (a) cytosolic and (b) nuclear fractions measured by western blotting. Three independent experiments were performed, and the data are presented as the mean ± SEM. *P<0.05 and **P<0.01 compared to the cells treated with LPS alone. Expressions of GAPDH and PCNA were used as internal controls for western blot analysis.