FIGURE

Fig. 1

ID

ZDB-FIG-210301-148

Publication

Zimmer et al., 2020 - Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)

Other Figures

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Fig. 1

Genotyping and confirmation of ca17a−/− deletion in larval zebrafish (Danio rerio). A: nucleotide alignment of the sequenced ca17a+/+ and ca17a−/− alleles showing the CRISPR sgRNA target sequence in the shaded box and the positions of the 7 nucleotide (nt) deletion and C to T substitution mutations in the red box. B: multiplex PCR products from fin clips of F2 larval offspring of F1 ca17a+/− crosses. The general amplicon (477 base pairs; bp) band appears for all genotypes, the 147-bp band is the ca17a+/+-specific amplicon, and the 352-bp band is the ca17a−/−-specific amplicon. C: representative Western blot of Ca17a and β-actin protein expression in ca17a+/+, ca17a+/−, and ca17a−/− whole larvae homogenates at 9 days postfertilization (dpf). D: immunofluorescent staining of Ca17a in the yolk sac of ca17a+/+, ca17a+/−, and ca17a−/− 4 dpf larval zebrafish. Scale bar = 50 μm.

Expression Data

Gene:	ca2
Fish:	WT ca2^ot206/ot206 ca2^ot206/+
Anatomical Terms:	whole organism yolk syncytial layer
Stage Range:	Day 4 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ca2^ot206/ot206 ca2^ot206/+
Observed In:	whole organism yolk syncytial layer
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.