Fig. 1
- ID
- ZDB-FIG-210301-148
- Publication
- Zimmer et al., 2020 - Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)
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Genotyping and confirmation of ca17a?/? deletion in larval zebrafish (Danio rerio). A: nucleotide alignment of the sequenced ca17a+/+ and ca17a?/? alleles showing the CRISPR sgRNA target sequence in the shaded box and the positions of the 7 nucleotide (nt) deletion and C to T substitution mutations in the red box. B: multiplex PCR products from fin clips of F2 larval offspring of F1 ca17a+/? crosses. The general amplicon (477 base pairs; bp) band appears for all genotypes, the 147-bp band is the ca17a+/+-specific amplicon, and the 352-bp band is the ca17a?/?-specific amplicon. C: representative Western blot of Ca17a and ?-actin protein expression in ca17a+/+, ca17a+/?, and ca17a?/? whole larvae homogenates at 9 days postfertilization (dpf). D: immunofluorescent staining of Ca17a in the yolk sac of ca17a+/+, ca17a+/?, and ca17a?/? 4 dpf larval zebrafish. Scale bar = 50 ?m. |
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Stage Range: | Day 4 to Days 7-13 |
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Observed In: | |
Stage Range: | Day 4 to Days 7-13 |