FIGURE

Fig. 4

ID

ZDB-FIG-210301-151

Publication

Zimmer et al., 2020 - Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)

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Fig. 4

Cl^? uptake rates and whole body Cl^? content of ca17a+/+, ca17a+/?, and ca17a?/? larvae (A and B), as well as sham Ca17a morpholino-treated larvae (C and D), and the effect of ethoxzolamide on Cl^? uptake (E) in zebrafish (Danio rerio) larvae. There was a significant effect of genotype on Cl^? uptake in 4- and 9-dpf larvae (A) (ANOVA; 4 dpf: F = 55.1, P < 0.01, n = 16 for ca17a+/+ and ca17a+/?, n = 12 for ca17a?/?; 9 dpf: F?=?21.5, P < 0.01, n = 13 for ca17a+/+, n = 12 for ca17a+/?, n = 11 for ca17a?/?) but not Cl^? content in 4 dpf (B) (ANOVA; F = 1.1, P = 0.36, n = 7 for ca17a+/+ and ca17a+/?, n = 6 for ca17a?/?) or 9 dpf (ANOVA; F?=?1.9, P = 0.18, n = 8 for ca17a+/+ and ca17a+/?, n = 5 for ca17a?/?) larvae. There was an effect of Ca17a knockdown on Cl^? uptake in 4-dpf zebrafish (C) (Welch?s two-sample t test; t?=?3.5, P < 0.01, n = 6), but not Cl^? content (D) (Welch?s two-sample t test; t = ?0.8, P = 0.5, n = 6). There was an effect of ethoxzolamide on Cl^? uptake in 4-dpf zebrafish (E) [one-way nonparametric ANOVA (Kruskal?Wallis); ?2?=?12.1, P < 0.01, n = 6]. Values with different letters are significantly different (P < 0.05) from one another. Data are presented as means � SE. dpf, days postfertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT ca2^ot206/ot206 WT + MO4-ca2
Condition:	chemical treatment by environment: ethoxzolamide
Knockdown Reagent:	MO4-ca2
Observed In:	whole organism chloride whole organism chloride transport
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

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