FIGURE

Figure 4—figure supplement 2.

ID

ZDB-FIG-210224-35

Publication

Xu et al., 2021 - Foxc1 establishes enhancer accessibility for craniofacial cartilage differentiation

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Figure 4—figure supplement 2.

In vivo validation of accessible elements from Group I.

(A) Genomic regions (gene loci and GRCz10 coordinates listed) for enhancer testing. Peaks (p) tested are shown in green, and μATACseq reads are shown in each row. DNCCs, dorsal CNCCs. (B) GFP expression (yellow) driven by the indicated peaks at 36 hpf and 6 dpf. For emx3_p1 and prrx1a_p1, confocal projections of whole arch CNCCs of stable transgenic zebrafish are shown in lateral view at 36 hpf. For satb2-p1, confocal section of representative injected embryo is shown in lateral view at 6 dpf, with DIC providing embryo context in white. Numbers indicate proportion of embryos positive for the co-selectable lens:CFP marker (also on the injected plasmid) in which the displayed patterns were observed. Scale bar = 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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