Figure 3—figure supplement 1.

Overlapping expression of <italic>foxc1a</italic> and <italic>foxc1b</italic> with <italic>pou3f3b>GFP</italic> in the dorsal-intermediate arches.

(A,B) In situ hybridization of foxc1a or foxc1b with pou3f3b:Gal4; UAS:nlsGFP (pou3f3b>GFP, detected by anti-GFP antibody) at 36 hpf. Dashed lines indicate regions of foxc1a or foxc1b expression, carried over to the pou3f3>GFP-only and merged images for reference. Scale bar = 100 μm. (C) Quantification of pou3f3b>GFP+; sox10:DsRed+ cartilage size in control and foxc1a–/–; foxc1b–/– mutant embryos at 6 dpf. Units were normalized to one based on the average size of the control cartilage. n indicates number of embryos analyzed. Shown is mean ± sem, and p value was calculated using Students’ T test (tails = 2, type = 3).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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