FIGURE

Figure 3

ID

ZDB-FIG-210206-16

Publication

Palominos et al., 2021 - The Olfactory Organ Is Populated by Neutrophils and Macrophages During Early Development

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Figure 3

The developing olfactory organs have an extensive blood-lymphatic system. Lyve1b:DsRed+ lymphatic vessels (red) at 5 dpf (A), 7 dpf (B), and 15 dpf (C). (A–C) Lyve1b:DsRed+ lymphatic vasculature (red, arrows) extends from the dorsal brain toward the olfactory organs (OO). (B,C) Lymphatic vessels extend to region of olfactory sensory neurons (OMP:YFP+), yellow, asterisks) in olfactory bulb (OB). (C) At 15 hpf, nasal lymphatic vasculature (red, arrowheads) is now visible wrapping around the posterior OO and associating with the ventral lateral OOs (G). Fli1a:EGFP+ blood vasculature (green) at 5 dpf (D), 7 dpf (E), and 15 dpf (F). Blood vessels (green) forming nasal vein (NV) and nasal ciliary artery (NCA) are present at 5 dpf. The NV extends ventrally (E) encircling the OO (F). (G) Diagram of head of 15 dpf larva showing telencephalon (green) and olfactory organs (gray). (G′) Olfactory organ (gray) summarizing blood (green) and lymphatic (red) vasculature. Nuclei of NV (orange) are positive for both lyve1b:DsRed and fli1a:EGFP. (A,D,E,F): DAPI (blue). OO: olfactory organ, OB: olfactory bulb, ey: eyes. Scale bars: (A–C) = 200 μm, (D–F) = 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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