Neutrophils and macrophages are found in developing sensory systems. (A) Frontal view of 7-dpf larvae. Bright-field/fluorescent image in whole-mount larvae with OMP:RFP+ OSNs. Lines in red indicate the future adult cribriform plate. (B) Frontal view, whole-mount 7-dpf Tg(mpx:GFP) larva, anti-Sox2–positive taste buds (red, arrowheads), neuromasts (red, asterisks), and neutrophils (green). (C) Lateral view, whole-mount 7 dpf larva. Neutrophils (green) associated with border of the ear (dashed line) (am: ampulla). (D) Neutrophils are highly represented in the olfactory organ (OO) with fewer neutrophils associated with the gustatory and auditory systems. No neutrophils were observed in the retina (n = 30 animals, one-way ANOVA, Tukey test, P < 0.0001). (E) Diagram of head of larva showing generalized position of the cartilages (red) giving rise to the cribriform plate in the adult animal. (F) Frontal view, whole-mount 7-dpf Tg(mpeg1:mCh) larvae, anti-HuC/D–positive neurons in the OO (pale blue), macrophages (red). (G) Lateral view, whole-mount 7 dpf. Macrophages (red) associated with border of the ear (dashed line). (H) The olfactory organ (OO) and the eye have the most macrophages, with fewer macrophages associated with gustatory (mo) and auditory (ear) systems (n = 30 animals, one-way ANOVA, Tukey test, P < 0.001, ns = non-significant). DAPI: blue, mo: mouth, tg: trigeminal ganglia. Scale bars: (B,C,F,G) = 100 μm.
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