Neutrophils and macrophages populate the olfactory system of juvenile animals. (A) Frontal view of Tg(mpx:GFP) 7dpf larva with anti-HuC/D–positive (red) neurons in the olfactory organ (OO) adjacent to the olfactory bulb (OB). (A′) Image of OO (boxed area in A) with neutrophils (green). (B) 25 μm cryosection of a 7-dpf Tg(OMP:RFP);Tg(mpx:GFP) larva neutrophils (green, asterisks) localized within the OO margin and adjacent to OSNs (red, see inset). (C) Average number (±SEM) of neutrophils in OOs of Tg(mpx:GFP) during the first 2 weeks postfertilization (n = 45 larvae). (D) Frontal view of Tg(mpeg1:mCh) 7 dpf larva. Anti-HuC/D–positive (green) neurons populate the olfactory organs (OO). (D′) Image of OO (boxed area in D) with macrophages (red) adjacent to the OO (arrow) and within the OO (arrowhead). (E) Lateral oblique view of OO at 7 dpf. (F) Average number (±SEM) of macrophages in OOs of Tg(mpeg1:mCh) during the first 2 weeks post-fertilization (n = 45 larvae). Scale bars: (A,D) = 100 μm; (A′,D′,E) = 50 μm, (B) = 25 μm.
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