FIGURE

Figure 2

ID

ZDB-FIG-210129-2

Publication

Vershinin et al., 2020 - PAK4 methylation by the methyltransferase SETD6 attenuates cell adhesion

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Methylated PAK4 at lysine 473 upregulates Wnt/β-catenin target genes. (A) Cell extracts of MDA-MB-231 cells stably expressing empty plasmid, Flag PAK4 wild-type or Flag PAK4 K473R mutant were subjected to Western blot to detect endogenous protein levels of β-catenin, active (non-phospho) β-catenin and β-catenin S675-ph. Uncropped gels are shown in Supplementary Fig. S9. (B) mRNA was extracted from MDA-MB-231 cells stably expressing empty plasmid, Flag PAK4 wild-type or Flag PAK4 K473R. Transcript levels of the indicated Wnt/β-catenin target genes were determined by qPCR. mRNA levels were normalized to GAPDH and then to empty cells. Error bars are s.e.m. Statistical analysis was performed for 3 experimental repeats using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.