Figure 2

Figure 2

Methylated PAK4 at lysine 473 upregulates Wnt/β-catenin target genes. (A) Cell extracts of MDA-MB-231 cells stably expressing empty plasmid, Flag PAK4 wild-type or Flag PAK4 K473R mutant were subjected to Western blot to detect endogenous protein levels of β-catenin, active (non-phospho) β-catenin and β-catenin S675-ph. Uncropped gels are shown in Supplementary Fig. S9. (B) mRNA was extracted from MDA-MB-231 cells stably expressing empty plasmid, Flag PAK4 wild-type or Flag PAK4 K473R. Transcript levels of the indicated Wnt/β-catenin target genes were determined by qPCR. mRNA levels were normalized to GAPDH and then to empty cells. Error bars are s.e.m. Statistical analysis was performed for 3 experimental repeats using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001.