FIGURE

Figure 5

ID

ZDB-FIG-210129-5

Publication

Vershinin et al., 2020 - PAK4 methylation by the methyltransferase SETD6 attenuates cell adhesion

Other Figures

All Figure Page

Back to All Figure Page

Figure 5

PAK4 K473me attenuates paxillin localization to focal adhesions. (A) MDA-MB-231 cells stably expressing empty plasmid, Flag PAK4 wild-type or Flag PAK4 K473R were fixed and stained with phalloidin (green), paxillin (red) and DAPI. Cells were visualized by a confocal microscope (63×). Scale bar 10 µm. Graph on the right represents the paxillin area size. Error bars are s.d. Statistical analysis was performed for n > 44 cells per condition using one-way ANOVA. ***p < 0.001. (B) MDA-MB-231 CRISPR SETD6 knock-out (SETD6 KO) cells were transfected with Flag PAK4 wild-type or Flag PAK4 K473R, with or without HA SETD6. Samples were immunoprecipitated with FLAG-M2 beads and submitted to Western blot with anti-paxillin antibody. Uncropped gels are shown in Supplementary Fig. S9.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.