FIGURE

Figure 7

ID

ZDB-FIG-200829-15

Publication

Hudson et al., 2020 - Berbamine Analogs Exhibit Differential Protective Effects From Aminoglycoside-Induced Hair Cell Death

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Figure 7

Berbamine analogs differentially reduce mechanotransduction channel activity. Mechanosensitive-calcium signals from hair bundles of posterior lateral line neuromasts. (A) Side view of a neuromast expressing hair cell-specific membrane-localized GCaMP6s. Left: an imaging plane through the apical hair bundles can be used to measure mechanosensitive-calcium signals during fluid-jet stimulation. Right: a top-down view of the imaging plane showing the hair bundles expressing GCaMP6s. (B) Traces comparing the average GCaMP6s signals before and after application of 1 μM BA-16, N = 10 neuromasts. (C) Dot plots quantifying the peak GCaMP6s response per neuromast. BA-16 does not alter evoked calcium activity in hair cells. (D) Traces comparing the average GCaMP6s signals before and after application of 1 μM BA-9, N = 7 neuromasts. (E) Dot plots quantifying the peak GCaMP6s response per neuromast. BA-9 reduces MET-evoked calcium activity compared to baseline levels. Each dot in (C,E) averages the response of all hair bundles in each neuromast. A paired t-test was used in (C,E), ***p < 0.001. Error bars in (C,E) and dashed lines on either side of the solid line in (B,D) represent ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.