Figure 7

Figure 7

Berbamine analogs differentially reduce mechanotransduction channel activity. Mechanosensitive-calcium signals from hair bundles of posterior lateral line neuromasts. (A) Side view of a neuromast expressing hair cell-specific membrane-localized GCaMP6s. Left: an imaging plane through the apical hair bundles can be used to measure mechanosensitive-calcium signals during fluid-jet stimulation. Right: a top-down view of the imaging plane showing the hair bundles expressing GCaMP6s. (B) Traces comparing the average GCaMP6s signals before and after application of 1 μM BA-16, N = 10 neuromasts. (C) Dot plots quantifying the peak GCaMP6s response per neuromast. BA-16 does not alter evoked calcium activity in hair cells. (D) Traces comparing the average GCaMP6s signals before and after application of 1 μM BA-9, N = 7 neuromasts. (E) Dot plots quantifying the peak GCaMP6s response per neuromast. BA-9 reduces MET-evoked calcium activity compared to baseline levels. Each dot in (C,E) averages the response of all hair bundles in each neuromast. A paired t-test was used in (C,E), ***p < 0.001. Error bars in (C,E) and dashed lines on either side of the solid line in (B,D) represent ± SEM.