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Figure 8

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ZDB-FIG-200829-16

Publication

Hudson et al., 2020 - Berbamine Analogs Exhibit Differential Protective Effects From Aminoglycoside-Induced Hair Cell Death

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Figure 8

Differential patterns of analog protection from gentamicin. Zebrafish were either pre-treated with the OPC of analog for 1 h, then co-treated with analog and gentamicin for 30 min (“pre-co” condition), co-treated with analog and gentamicin without a pretreatment period (“co” condition) or post-treated with analog for 5.5 h following gentamicin removal (“post” condition). All treatments used 200 μM gentamicin. Examples are shown for analogs that represent each pattern of protection. (A) Analog BA-1 was only protective when administered in the “pre-co” or “co” conditions suggesting that protection occurs via aminoglycoside uptake block. (B,C) Analogs BA-6 and BA-7 significantly protected hair cells in all three conditions, suggesting an intracellular mechanism of protection in addition to aminoglycoside uptake block. (D) Analog BA-16 is not protective of any treatment paradigm. Hair cells were assessed via DASPEI. Data are presented normalized to untreated controls, which are not shown but represent 100% hair cell survival. Data were analyzed via one-way ANOVA, ****p < 0.0001, **p < 0.01 (as compared to gentamicin-only controls), N = 7–12, bars are + SEM. Refer to Supplementary Table S1 for statistics.

Expression Data

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.