ZFIN ID: ZDB-FIG-200608-26
Tyrkalska et al., 2019 - Inflammasome Regulates Hematopoiesis through Cleavage of the Master Erythroid Transcription Factor GATA1. Immunity   51(1):50-63.e5 Full text @ Immunity
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Days 7-13

Fig. 4 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gata1a mitfaw2/w2; mpv17a9/a9 + MO1-gbp4 control Prim-5 whole organism RTPCR
mitfaw2/w2; mpv17a9/a9 + MO4-pycard control Prim-5 whole organism RTPCR
spi1b mitfaw2/w2; mpv17a9/a9 + MO1-gbp4 control Prim-5 whole organism RTPCR
mitfaw2/w2; mpv17a9/a9 + MO4-pycard control Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-gata1a WB mitfaw2/w2; mpv17a9/a9 + MO1-gbp4 control Prim-5 whole organism
WB mitfaw2/w2; mpv17a9/a9 + MO2-gata1a control Prim-5 whole organism
WB mitfaw2/w2; mpv17a9/a9 + MO4-pycard control Prim-5 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
i114Tg chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor Protruding-mouth cysteine-type endopeptidase activity involved in apoptotic process decreased occurrence, abnormal
Protruding-mouth neutrophil decreased amount, abnormal
i114Tg chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor, bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth cysteine-type endopeptidase activity involved in apoptotic process decreased occurrence, abnormal
Protruding-mouth neutrophil decreased amount, abnormal
i114Tg bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth cysteine-type endopeptidase activity involved in apoptotic process increased occurrence, abnormal
Protruding-mouth neutrophil increased amount, abnormal
i114Tg + MO4-pycard control Protruding-mouth cysteine-type endopeptidase activity involved in apoptotic process decreased occurrence, abnormal
Protruding-mouth neutrophil decreased amount, abnormal
mitfaw2/w2; mpv17a9/a9 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 - Days 7-13 whole organism decreased life span, abnormal
mitfaw2/w2; mpv17a9/a9 + MO1-gbp4 control Prim-5 whole organism gata1a expression increased amount, abnormal
Prim-5 whole organism spi1b expression decreased amount, abnormal
Prim-5 whole organism Ab2-gata1a labeling increased amount, abnormal
mitfaw2/w2; mpv17a9/a9 + MO2-gata1a control Prim-5 whole organism Ab2-gata1a labeling decreased amount, abnormal
mitfaw2/w2; mpv17a9/a9 + MO4-pycard control Prim-5 whole organism gata1a expression increased amount, abnormal
Prim-5 whole organism spi1b expression increased amount, abnormal
Prim-5 whole organism Ab2-gata1a labeling increased amount, abnormal
mitfaw2/w2; mpv17a9/a9 + MO4-pycard bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 - Days 7-13 whole organism decreased life span, exacerbated
Full text @ Immunity