Figure 1—figure supplement 4

(A) Expression of YSL marker genes (slc26a1, slc40a1, mxtx2, gata6 and camsap1l1) in stage-matched embryos (side view) and blastoderm explants (side view), as determined by whole mount in situ hybridization at 50% epiboly. The proportion of embryos or blastoderm explants with a phenotype similar to the images shown is indicated in the lower right corner (slc26a1: embryos, n = 29, N = 3, explants, n = 38, N = 3; slc40a1: embryos, n = 21, N = 3, explants, n = 26, N = 3; mxtx2: embryos, n = 24, N = 4, explants, n = 28, N = 4; gata6: embryos, n = 24, N = 3, explants, n = 29, N = 3; camsap1l1: embryos, n = 23, N = 3, explants, n = 29, N = 3). (B) Percentage of blastoderm explants showing normal, strongly reduced or no expression of YSL marker genes (slc26a1: n = 38, N = 3; slc40a1: n = 26, N = 3; mxtx2: n = 28, N = 4; gata6: n = 29, N = 3; camsap1l1: n = 29, N = 3) as determined by whole mount in situ hybridization at 50% epiboly. (C) High-resolution fluorescence images of a blastoderm explant at 50% epiboly (top views shown at different optical sections). Cell membranes (grey) are marked by lyn::tdTomato and nuclei (green) by h2b::EGFP. The proportion of blastoderm explants with a phenotype similar to the images shown is indicated in the lower right corner (n = 14, N = 3). Yellow circle denotes the wounding site. Scale bars: 200 µm (A), 100 µm (C).

Figure 1—figure supplement 4—source data 1.