Figure 2—figure supplement 2

(A) Schematic representation of the injection method used to label a single marginal cell in 128 c embryos. (B) High-resolution fluorescence images of a blastoderm explant (top view) from late sphere stage onwards (n = 10, N = 3). Cell membranes (grey) are marked by Membrane-RFP and injected cells (grey) by H2A-chFP expression. Time in min. The explant and clone edges are outlined with a white or yellow dashed line, respectively. (C) Average normalized distance of positive cells (marked by H2A-chFP expression) to the clone center from sphere stage onwards (n = 10, N = 3). (D) Average normalized distance between positive cells (marked by H2A-chFP expression) from sphere stage onwards (n = 10, N = 3). (E) Expression of chrd (embryos, n = 20, N = 3; explants, n = 20, N = 3) and bmp2b (embryos, n = 24, N = 3; explants, n = 41, N = 3) in stage-matched embryos and blastoderm explants at 50% epiboly, as determined by whole mount in situ hybridization. The view shown for both embryos and blastoderm explants is indicated at the top. The proportion of embryos or blastoderm explants with a phenotype similar to the images shown is indicated in the lower right corner. (F) High-resolution fluorescence images of stage-matched embryos (lateral view) and blastoderm explants (top view) expressing gsc::GFP-CAAX stained both for pSMAD1/5 (purple) and DAPI (grey) at 50% epiboly (embryos: n = 13, N = 4; explants: n = 29, N = 4). Nuclear pSMAD1/5 is color-coded using a fire lookup table (highest intensities in yellow) and was masked based on the DAPI signal. Insets are zoom-in images of the highlighted regions (dashed boxes). Yellow circle denotes the wounding site in the explant. Scale bars: 100 µm (B,F), 200 µm (E).

Figure 2—figure supplement 2—source data 1.