ZFIN ID: ZDB-FIG-200314-74
Kang et al., 2020 - Global Transcriptomic Analysis of Zebrafish Glucagon Receptor Mutant Reveals Its Regulated Metabolic Network. International Journal of Molecular Sciences   21(3) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Figure 7

Validation of RNA-seq data using qPCR. (A–D) The validation of the expression levels of differentially expressed genes by qRT-PCR analysis in the categories of lipid metabolism (A) carbohydrate metabolism, (B) amino acid metabolism, (C) and other DEGs (D). (E) Correlation between qRT-PCR and RNA-seq results for select DEGs. The log2 (fold change) values derived from the RNA-seq analysis of DEGs are compared with those obtained by qRT-PCR determined by 2−ΔΔCT. The reference line indicates the expected linear relationship.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acss2l gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
adh5l gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
apobb.2 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
cox11 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
cthl gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
dnmt1 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
eevs gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
elovl7b gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
fasn2 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
fcsk gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
gale gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
gcgb gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
hadhaa gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
il4i1 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
lipca gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
mlycd gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
ndufs8b gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
papss1 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
pcsk9 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
pvalb3 gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
slc2a1a gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gcgravu600/vu600 ; gcgrbvu601/vu601 standard conditions Days 7-13 whole organism acss2l expression increased amount, abnormal
Days 7-13 whole organism adh5l expression increased amount, abnormal
Days 7-13 whole organism apobb.2 expression decreased amount, abnormal
Days 7-13 whole organism cox11 expression decreased amount, abnormal
Days 7-13 whole organism cthl expression decreased amount, abnormal
Days 7-13 whole organism dnmt1 expression decreased amount, abnormal
Days 7-13 whole organism eevs expression decreased amount, abnormal
Days 7-13 whole organism elovl7b expression increased amount, abnormal
Days 7-13 whole organism fasn2 expression decreased amount, abnormal
Days 7-13 whole organism fcsk expression decreased amount, abnormal
Days 7-13 whole organism gale expression decreased amount, abnormal
Days 7-13 whole organism gcgb expression increased amount, abnormal
Days 7-13 whole organism hadhaa expression decreased amount, abnormal
Days 7-13 whole organism il4i1 expression decreased amount, abnormal
Days 7-13 whole organism lipca expression increased amount, abnormal
Days 7-13 whole organism mlycd expression decreased amount, abnormal
Days 7-13 whole organism ndufs8b expression decreased amount, abnormal
Days 7-13 whole organism papss1 expression decreased amount, abnormal
Days 7-13 whole organism pcsk9 expression decreased amount, abnormal
Days 7-13 whole organism pvalb3 expression decreased amount, abnormal
Days 7-13 whole organism slc2a1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.