(A–C) Effects of specialized diets, having no restriction (control diet (CD), black), 25% (blue) or 50% (red) calorie restriction (CR), on viability of zebrafish; solid line, frnt mutant; dotted line, control fish. (A–B) The effect of the specific dietary reduction in calories on survival in frnt; 50%CR lead to significant increase in survival of mutant and siblings compared to control and 25%CR; differences between 50%CR diets for both mutant and siblings are all significant (p<0.001) compared to control and 25%CR by Mantel-Cox and Geha-Brelow-Wilcoxon tests. (C–E) Caloric restriction ameliorates aberrant swimming behavior in celsr1a mutants. (C) High frequency turning behavior of mutants in tank (arrows). Quantitation of change in direction (D) and erratic turns (E) in treatment groups with different levels of caloric reduction. (F–H) Expression of genes associated with senescence and lifespan in brain tissue of wild type and celsr1a mutants in different dietary treatments: (F) sirt1; (G) sirt6 and (H) cdnk1a/p21; data represented as mean ± standard deviation. (I–J) Effect of caloric restriction on slow muscle fiber area in wild-type and celsr1a mutant adult zebrafish. (I) Slow muscle fiber area is potentiated in response to 50%CR in both wild-type and mutants. (J) sirt1 expression in slow muscle from different diet treatment groups. (K–M), Effect of caloric restriction on intestinal differentiation phenotypes of wild-type celsr1a mutant adults. (K) goblet cell number quantitated from different regional areas of the gut in CD and 50%CR treatment groups. Data presented as mean + /- standard deviation, *p<0.05, paired t-test, n = 4 (frnt), n = 3 (sibs). (L) Goblet cell morphology in CD and 50%CR treated mutant posterior intestine.
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