FIGURE

Figure 3

ID

ZDB-FIG-200215-32

Publication

Li et al., 2020 - Celsr1a is essential for tissue homeostasis and onset of aging phenotypes in the zebrafish

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Figure 3

<italic>frnt</italic> mutation does not affect senescence biomarkers.

(A) Southern blot of genomic DNA of zebrafish telomerase reverse transcriptase (tert) and frnt mutants probed for telomere repeats. Data shows no effect of the mutation on telomere length in the frnt mutant compared with significant decrease of intensity and size of telomere in tert deficient fish. Arrow points to upper size expected for zebrafish telomeres; n = 4 for each group. (B–E). Senescence-associated beta-galactosidase (SA-βgal) staining (blue) of frnt mutants. (B, C) Positive signal of senescence as shown in 5 month old tert mutants (C), n = 3) compared with their heterozygous controls (B), n = 3). Comparably staged wild-type and frnt adults (E), n = 4) show resting levels of SA-βgal signal. Samples were counterstained with nuclear red.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	celsr1a^{t31786/t31786} (TU) tert^{hu3430/hu3430} (TU)
Observed In:	telomere maintenance via telomerase
Stage:	Adult

Phenotype Detail

Acknowledgments

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