Ultrastructural properties of BLECs/LLECs are conserved in zebrafish and mice. a Representative TEM micrograph of adult zebrafish meninges. The large, round, densely and homogenously-stained inclusions (yellow asterisks) of a putative BLEC are indicated. Scale bar = 2.5 µm (in all images). n = 2 brains. b Representative TEM micrograph of adult mouse leptomeninges. Putative LLECs have their large inclusions marked with yellow asterisks. n = 2 brains. c Cells with large inclusions (yellow asterisks) characteristic of BLECs are immunologically labelled with post-embed detection of GFP in Tg(flt4:gfp) adult brains using a 20 nm gold-labelled secondary. Minimal contrast staining was used to prevent obscuring of the nanogold particles (highlighted in magenta). n = 2 brains. d Mouse meningeal cells with large inclusions (yellow asterisks) are labelled by pre-embed detection of LYVE1 using a nanogold secondary with silver-enhancement. The silver grains are highlighted with magenta. Note the LYVE1-positive cell has silver grains present near the outer membrane. n = 2 brains. e An example of a GFP-negative cell in zebrafish Tg(flt4:gfp) adult meninges with similar ultrastructural properties to macrophages. n = 2 brains. f A second population of LYVE1-positive cells in the mouse leptomeninges contain multiple small, irregular, heterogeneous vesicles with silver grains (magenta) present within the cytoplasm and vesicles. These cells are ultrastructurally similar to macrophages (e). n = 2 brains
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