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PRL-3 modulates Src phosphorylation.a Representative western blot analysis of Src_pY527, total Src, and CSK in Jurkat cells with PRL-3 knock-down. b Western blot validation of Src pathway in PRL-3 overexpressing Jurkat cells. Cells were serum starved overnight and added to serum containing complete media for the indicated time points. Numbers shown represent relative protein expression. c Representative western blot validation of Src pathway in 3xFlag PRL-3 Wt and 3xFlag PRL-3 C104S mutant Jurkat cells. d Quantification of n = 4 independent experiments analyzing SRC_pY527, *p = 0.003. e Co-immunoprecipitation assay of Jurkat overexpressing PRL-3 substrate trapping mutants, 3xFlag PRL-3 C104S or 3xFlag PRL-3 C104D, did not pulldown Src. f Schematic of PRL-3 and modulation of Src pathway in T-ALL cells.
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