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ZFIN ID: ZDB-FIG-191230-628
Zhao et al., 2019 - Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition. Cell Research   29(11):895-910 Full text @ Cell Res.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7

PIP3 reduction is mainly caused by PLCγ mediated PIP2 hydrolysis. Representative confocal images (a) and quantitative analysis (b) of trunk vessel phenotypes of WT or cds2 mutant embryos with vegfa OE and with or w/o plcg1 MO. The ISV number counted from 20–22 embryos per group is shown on the top (b). c Relative vegfa mRNA level of cds2 mutants with vegfa OE injected with ctrl or plcg1 MO. vegfa expression level was determined at 2 h post heatshock induction and normalized to WT embryos without vegfa OE. n = 3 samples, 15–20 embryos pooled for each sample. dplcg1 knockdown partially restored PIP2 and PIP3 level in cds2-deficient endothelium with vegfa OE. Western blotting analysis on a-Tubulin serves as the internal control for cell amounts in each sample collected during lipid quantitation analysis. n = 4 samples per group. e Working model of VEGFA-triggered vessel regression on CDS2-deficient endothelium. The outcome of VEGFA signaling can be reversed from angiogenesis to vessel regression, which is dependent on CDS2-controlled PIP2 and PIP3 availability and FOXO1 signaling activation. Scale bar, 100 μm. Error bars, mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant (P ≥ 0.05). See also Supplementary information, Fig. S8

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cds2cas008/cas008; y1Tg; zf1092Tg heat shock Protruding-mouth intersegmental vessel cell migration involved in sprouting angiogenesis process quality, abnormal
Protruding-mouth intersegmental vessel malformed, abnormal
Protruding-mouth intersegmental vessel retracted, abnormal
cds2cas008/cas008; y1Tg; zf1092Tg + MO4-plcg1 heat shock Protruding-mouth intersegmental vessel cell migration involved in sprouting angiogenesis process quality, ameliorated
Protruding-mouth intersegmental vessel malformed, ameliorated
Protruding-mouth intersegmental vessel retracted, ameliorated
y1Tg + MO2-cds2 control Long-pec whole organism 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate decreased amount, abnormal
y1Tg; zf1092Tg + MO2-cds2 heat shock Long-pec whole organism 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate decreased amount, abnormal
Long-pec whole organism 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate decreased amount, abnormal
y1Tg; zf1092Tg + MO2-cds2 + MO4-plcg1 heat shock Long-pec whole organism 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate decreased amount, ameliorated
Long-pec whole organism 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate decreased amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell Research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Res.