Effect of mannitol/glucose treatment with/withoutsodium nitroprusside(SNP) onglial fibrillary acidic protein(GFAP) expression. (A) Representative micrographs showing the effect of mannitol/glucose exposure with or without SNP treatment on GFAP expression (red channel represents GFAP; blue channel represents DAPI). Scale bars: 20 µm. (B) Quantification of GFAP expression in the optic tectum (n=16, 12, 18 and 20 larvae for mannitol, mannitol+SNP, glucose and glucose+SNP, respectively). Data are mean±s.e.m. *P<0.05, ***P<0.001.
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