Effect of mannitol/glucose treatment with/withoutsodium nitroprusside(SNP) onnitric oxide(NO) reactivity, quantified by intensity of DAF-FM staining. (A) Representative micrographs of tectal vessels showing separate and merged channels (green: DAF-FM staining; red: kdrl:HRAS-mCherry) for 20 mM mannitol- or glucose-exposed larvae co-treated with or without SNP. Scale bars: 20 µm. (B) Quantification of DAF-FM intensity in the tectal vessels (n=25, 24, 27 and 24 larvae for mannitol, mannitol+SNP, glucose and glucose+SNP, respectively). Data are mean±s.e.m. *P<0.05.
|