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ZFIN ID: ZDB-FIG-191230-446
Tuttle et al., 2019 - Retrograde Ret signaling controls sensory pioneer axon outgrowth. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-15 to Day 5

Figure 5 In vivo imaging of Ret51 trafficking.

(A) Kymographs generated from the pLLG pioneer axons of embryos injected with BAC expressing GFP-tagged zebrafish Ret51. (B) Kymograph generated from the pLLG axons of embryos injected with plasmid expressing mCherry-tagged RET51. Discrete tagged particles of Ret51 are trafficked in both cases anterogradely and retrogradely.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ret IHC mapk8ip3nl7/nl7; nl1Tg transection: axon: posterior lateral line ganglion Day 5 posterior lateral line nerve regenerating tissue
IHC nl1Tg transection: axon: posterior lateral line ganglion Day 5 posterior lateral line nerve regenerating tissue
Ab2-ret IHC mapk8ip3nl7/nl7; nl1Tg transection: axon: posterior lateral line ganglion Day 5 posterior lateral line nerve regenerating tissue
IHC nl1Tg transection: axon: posterior lateral line ganglion Day 5 posterior lateral line nerve regenerating tissue
Phenotype Details
Fish Conditions Stage Phenotype
mapk8ip3nl7/nl7; nl1Tg control Prim-15 posterior lateral line ganglion retrograde axonal protein transport decreased occurrence, abnormal
mapk8ip3nl7/nl7; nl1Tg transection: axon: posterior lateral line ganglion Day 5 posterior lateral line nerve regenerating tissue Ab2-ret labeling decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife