FIGURE

Figure 4 supplement 1

ID

ZDB-FIG-191230-445

Publication

Tuttle et al., 2019 - Retrograde Ret signaling controls sensory pioneer axon outgrowth

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Figure 4 supplement 1

Anti-pJNK antibody immunostaining of pioneer growth cones.

(A,B) Immunostaining for phosphorylated JNK in pioneer axons in wild type (A) and ret^hu2846 mutants (B) at 30 hpf. pJNK signal in extending pioneer growth cones looks similar between siblings (A’) and mutants (B’). (C) Mean fluorescence intensity measured for the most distal pLLG growth cones (visualized by neurod:EGFP transgene) was compared between sibling (306 ± 26, n = 14) and ret^hu2846 mutants (290 ± 51, n = 6) at 30 hpf, as not significantly different (p=0.78 by Mann-Whitney U test). Error bars represent S.E.M.

Expression Data

Antibody:	Ab15-mapk
Fish:	nl1Tg ret^{hu2846/hu2846}; nl1Tg
Anatomical Term:	posterior lateral line ganglion growth cone
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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