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ZFIN ID: ZDB-FIG-191230-444
Tuttle et al., 2019 - Retrograde Ret signaling controls sensory pioneer axon outgrowth. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Figure 4 Anti-pJNK antibody immunostaining of pioneer growth cones.

(A,B) Immunostaining for phosphorylated JNK in pioneer axons in wild type (A) and rethu2846 mutants (B) at 30 hpf. pJNK signal in extending pioneer growth cones looks similar between siblings (A’) and mutants (B’). (C) Mean fluorescence intensity measured for the most distal pLLG growth cones (visualized by neurod:EGFP transgene) was compared between sibling (306 ± 26, n = 14) and rethu2846 mutants (290 ± 51, n = 6) at 30 hpf, as not significantly different (p=0.78 by Mann-Whitney U test). Error bars represent S.E.M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mapk8ip3nl7/nl7; nl1Tg standard conditions Prim-15 posterior lateral line ganglion growth cone IHC
nl1Tg standard conditions Prim-15 posterior lateral line ganglion growth cone IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-ret IHC mapk8ip3nl7/nl7; nl1Tg standard conditions Prim-15 posterior lateral line ganglion growth cone
IHC nl1Tg standard conditions Prim-15 Not Detected posterior lateral line ganglion growth cone
Ab2-ret IHC mapk8ip3nl7/nl7; nl1Tg standard conditions Prim-15 posterior lateral line ganglion growth cone
IHC nl1Tg standard conditions Prim-15 Not Detected posterior lateral line ganglion growth cone
Phenotype Details
Fish Conditions Stage Phenotype
mapk8ip3nl7/nl7; nl1Tg standard conditions Prim-15 posterior lateral line ganglion growth cone ab1-ret labeling increased amount, abnormal
Prim-15 posterior lateral line ganglion growth cone Ab2-ret labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife