ZFIN ID: ZDB-FIG-191230-1705
Brenet et al., 2019 - Defective Excitatory/Inhibitory Synaptic Balance and Increased Neuron Apoptosis in a Zebrafish Model of Dravet Syndrome. Cells   8(10) Full text @ Cells
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Figure 1

Correlation between LFP and calcium activity in scn1Lab model. (A) Schematic of the experimental setup to simultaneously record local field potential and calcium activity of a larval zebrafish brain. (B,C) Calcium images showing respectively the baseline activity (B) and the seizure activity (C). The fluorescence intensity is color-coded as shown in the color bar below the images. (D,E) Representative 20 min calcium recording of immobilized and paralyzed 4 dpf control (D) (N = 9) and scn1Lab (E) (N = 9) larvae. Each calcium event is shown with an orange arrowhead. (F) Number of events, calcium increases greater that 0.04 ΔF/F0 amplitude, during 1 h recording. (G) Representative LFP, recorded in the left neuropil of the optic tectum, and calcium traces, from the optic tectum, of a paralyzed and immobilized 4 dpf scn1Lab larva. (H) Correlation between LFP and calcium events duration (n = 46). N = number of larvae and n = number of events. Error bars on all graphs represent standard error of the mean (SEM). ***, p < 0.001. p-value was determined using Student’s unpaired t-test.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf498Tg + MO2-scn1lab standard conditions Day 4 calcium-mediated signaling increased occurrence, abnormal
Day 4 neuronal signal transduction amplitude, abnormal
Day 4 neuronal signal transduction increased occurrence, abnormal
Acknowledgments:
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