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Defects of excitatory/inhibitory balance in the Scn1Lab-depleted larvae. (A,B) 20 µm coronal sections of 5 dpf control (A) (N = 5, n = 15) and scn1Lab (B) (N = 5, n = 15) larvae stained with PSD-95, an excitatory post-synaptic scaffolding protein. (D,E) 20 µm coronal sections of 5 dpf control (D) (N = 5, n = 20) and scn1Lab (E) (N = 5, n = 19) larvae stained with gephyrin, an inhibitory post-synaptic scaffolding protein. Images were acquired with a SP8 Leica laser scanning confocal microscope equipped with a 40x/oil 1.3 objective. Scale bar 5 µm. (C,F) Quantification of PSD-95 (C) and gephyrin (F) puncta density. (G) PSD-95/gephyrin puncta density ratio. N = number of larvae and n = number of sections. Error bars on all graphs represent standard error mean (SEM). **, p < 0.01. p-values were determined using the Mann-Whitney test.
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