Figure 1

Figure 1

Correlation between LFP and calcium activity in scn1Lab model. (A) Schematic of the experimental setup to simultaneously record local field potential and calcium activity of a larval zebrafish brain. (B,C) Calcium images showing respectively the baseline activity (B) and the seizure activity (C). The fluorescence intensity is color-coded as shown in the color bar below the images. (D,E) Representative 20 min calcium recording of immobilized and paralyzed 4 dpf control (D) (N = 9) and scn1Lab (E) (N = 9) larvae. Each calcium event is shown with an orange arrowhead. (F) Number of events, calcium increases greater that 0.04 ΔF/F₀ amplitude, during 1 h recording. (G) Representative LFP, recorded in the left neuropil of the optic tectum, and calcium traces, from the optic tectum, of a paralyzed and immobilized 4 dpf scn1Lab larva. (H) Correlation between LFP and calcium events duration (n = 46). N = number of larvae and n = number of events. Error bars on all graphs represent standard error of the mean (SEM). ***, p < 0.001. p-value was determined using Student’s unpaired t-test.